Okay, my understanding is that you want calls to ring all phones, but if no one is available go directly to application 2541, right? It's possible to do exactly that.
I assume you already have an extension list with all those members in it (if not, create one). Be sure HG is set up for LINEAR distribution, which will the first list entry if available, skip to second if not. For members make the extension list the first entry, app 2541 the second. Now when a call arrives at the HG it will ring all available members first, then time out to second list entry, 2541 (set by NO ANSWER ADVANCE timer). If no one on the list is available it will skip that "member" and go directly to 2541. But you probably don't have the mind-reading-module available, so its important for agents to tell the system they're not available by going to DND or using the HUNTGROUP REMOVE/REPLACE feature code or button.
If instead you literally want line 2 to always ring directly to 2541 you need to build a second trunk group for that line and set ring in to 2541.